The derivation of membranous epithelial (M) cells, which are specialized antigen sampling cells overlying the lymphoid follicles of Peyer's patches, is unknown; it has been suggested recently, however, that M cells differentiate from absorptive cells on the follicular epithelium. To examine whether M cells, like other intestinal epithelial cells, derive directly from undifferentiated crypt cells, we studied the structure, selected functional features, proliferation, and distribution of Peyer's patch M cells in the ileum of adult mice. We observed a spectrum of M-cell structure ranging from mature to immature-appearing M cells. Most immature-appearing M cells lacked the central cytoplasmic hollow found in those mature M cells that contained lymphoid cells. Download Brian Jonestown Massacre Methodrone Rar. The microvilli of immature-appearing M cells were more numerous and regular appearing than those of mature M cells, but they were sparser and shorter, and some were wider than those of absorptive cells.

Many immature-appearing M cells contained more free ribosomes than did mature M cells. Both mature and immature-appearing M cells were observed on all regions of follicular domes, including the base near the mouths of surrounding crypts. Type 1 reovirions adhered with considerable selectivity to the apical membrane of mature and immature-appearing M cells but were observed in endocytic vesicles only in mature M cells. Neither mature nor immature-appearing M cells showed evidence of lipid absorption, in contrast to adjacent absorptive cells. Both mature and immature-appearing M cells internalized more bound cationized ferritin than did absorptive cells. Only mature M cells transported cationized ferritin to the intercellular spaces. Nuclei of a few immature-appearing M cells were labeled 24 h after injection of [3H]thymidine in concert with the appearance of labeled absorptive cell nuclei.

These observations strongly suggest that many, if not all, M cells derive directly from undifferentiated crypt cells.

Jun 15, 2008 Comprehensive gene expression profiling of Peyer's patch M cells, villous M-like cells, and intestinal epithelial cells. Terahara K(1), Yoshida M, Igarashi O, Nochi T, Pontes GS, Hase K, Ohno H, Kurokawa S, Mejima M, Takayama N. Peyer’s Patch M Cells Derived from Lgr5 Stem Cells Require SpiB and Are Induced by RankL in Cultured “Miniguts” Wim de Lau,a Pekka Kujala,b Kerstin Schneeberger. Sep 19, 2010 Fujimura Y. Functional morphology of microfold cells (M cells) in Peyer’s patches: phagocytosis and transport of BCG by M cells into rabbit Peyer’s. Isolation and in vitro Activation of Mouse Peyer’s Patch Cells from Small Intestine Tissue Reviewed. Andrea Introini Kathrin Sutter. 70 µm cell strainer. Blitzkrieg Commander Rulebook Pdf To Excel.